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Flow Cytometry

Northwestern University › Robert H. Lurie Comprehensive Cancer Center › Flow Cytometry - Instrumentation

 

Robert H. Lurie Comprehensive Cancer Center
Beckman Coulter Epics XL-MCL

Beckman Coulter Epics XL-MCL:  The Epics XL is a four-color capable bench-top flow cytometry analyzer fitted with a 15mW air cooled argon ion laser. A multi-tube carousel loader (32 tubes) is also mated to the instrument to permit walk away operation for routine types of analysis with large numbers of samples. This instrument is used primarily for routine flow cytometric assays, and for sophisticated assays which do not require flow cytometric sorting or multiple lasers. Investigators are encouraged to learn the operation of this cytometer to acquire and analyze their own work, under the guidance of facility staff.

Beckman Coulter Elite ESP:  The Elite ESP flow cytometer/cell sorter is fitted with two lasers, a 20mW 488 argon laser and a 10mW helium neon (HeNe) laser. Both lasers can be utilized simultaneously and with the adaptable filter configurations, up to five color analysis can be performed. The Elite is a two-way droplet cell sorter that runs at lower pressures and speeds than the MoFlo. Beckman Coulter Elite ESP
DakoCytomation MoFlo DakoCytomation MoFlo:  The MoFlo is a high speed multilaser droplet cell sorter. This system has enhanced multilaser capabilities (four separate laser interrogation spots) and is fitted with five lasers - excitation lines ranging from 350nm to 647nm. Due to the wide range of excitation wavelengths, most available fluorescent probes utilized in flow cytometry can be accommodated. The MoFlo will sort 10-15 times faster than the Elite and has four-way and single-cell sorting capabilities.
DakoCytomation CyAn:  The Cyan is a benchtop multilaser flow cytometer equipped with three lasers - excitation lines at 488nm, 635nm and 405nm (violet) wavelengths. It has enhanced multilaser analysis capabilities with three laser interrogation spots and nine-color capability. DakoCytomation CyAn
     

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Flow Cytometry Core Facility  710 N. Fairbanks Court  Chicago, IL  60611
Phone:  (312) 908-1294   Fax:  (312) 908-1372   E-mail:  J-Nelson3@northwestern.edu

Last updated November, 2007  World Wide Web Disclaimer and University Policy Statements
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