Flow Cytometry
Northwestern University › Robert H. Lurie Comprehensive Cancer Center › Flow Cytometry-First Time Users
Info for First Time Users:
Prior to commencing use of the facility, investigators are encouraged to meet with the facility manager to discuss experiment plans. As the facility is now offering sample preparation as an additional service, investigator involvement can range from non existent to very “hands on”. Investigators planning to use the benchtop flow cytometers (Beckman Coulter XL-MCL or Dako-Cytomation CyAn) are encouraged to learn the operation of the instrument; training is provided. For those who are unable to do so, data collection will be performed by the facility personnel. It is the responsibility of the Core lab to perform instrument quality control and maintenance. However, each investigator is responsible for the quality control of the application being run, review of the data produced, and ultimately, the final data analysis. Investigators wishing to initiate a flow cytometry based project should contact the core facility via phone, (312) 908-1294, or e-mail, J-marvin@northwestern.edu .
Data Analysis Options / Data File Maintenance:
In addition to the instrument data collection and analysis software, the core facility has additional software packages, which can be used for data analysis. These are Modfit (Verity Software House) for cell cycle distribution analysis, Expo (Applied Cytometry Systems) and Winlist (Verity Software House) for listmode data analysis, and Powerpoint for graphics production. Listmode data files are archived on CD-ROM by the facility personnel. Investigators may bring their own media for data transfer (zip disk, recordable CD-ROM) for off site analysis of data files. Facility staff will train investigators in the use of the data analysis software programs for the purpose of data reanalysis or graphics production. Alternatively, should the staff be requested to perform the work, a fee will be assessed (see Fees Table ).
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Flow Cytometry Core Facility 710 N. Fairbanks Court Chicago, IL 60611
Phone: (312) 908-1294 Fax: (312) 908-1372 E-mail: J-Nelson3@northwestern.edu
Last updated November, 2007 World Wide Web Disclaimer and University Policy Statements
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