Protocols
Below are
frequently requested protocols for some of the more commonly
performed flow cytometry assays. Core Facility personnel
are willing to assist investigators in implementing any
flow cytometry protocol seen in the literature and/or work with
investigators interested in assay development.
Commercial
kits are available for numerous assays (Annexin V, TUNEL, BrdU,
etc.). We have had experience with many of these kits and
will assist investigators with the use of any available commercial
kit.
Please
note that all of the protocols below have aspects which may need
to be optimized for any given cell system before ideal results
are obtained.
For optimal results see the "Technical Tips" section
for troubleshooting individual procedures.
