Figure 7:  Apoptotic regulatory protein detection:  B-CLL cells assayed fresh or after being placed in short term culture.  Cells then stained for phenotypic markers and fixed/permeabilized (Intraprep Permeabilization Reagent, Immunotech).  Cells then labeled with Bcl-2 and Bax (a; above), or the active subunit of Caspase-3 and Bax (b; below).