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Flow Cytometry

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Robert H. Lurie Comprehensive Cancer Center



CELL SORTING

The Robert H. Lurie Comprehensive Cancer Center Flow Cytometry Core Facility offers cell sorting to investigators seeking to isolate a subpopulation of cells for further study/analysis, including sterile sorting for subsequent subculture.  For investigators coming from off-site locations, the facility houses a CO2 incubator, cell culture hood, and centrifuge for on-site cell manipulation if necessary.  The cell sorting can be performed by two of our instruments-the Beckman Coulter Elite ESP and the DakoCytomation MoFlo.  Choice of sorting service can be determined upon discussion with core facility personnel and will depend upon experiment design and cell yield requirements.  A brief overview of the cell sorting capabilities is as follows:


Beckman Coulter Elite ESP:

  • Low pressure, i.e. 8psi, cell sorting (required for some cell types)

  • Cell sorting at rates of approximately 2000 cells/second

  • Two-way sorting (2 subpopulations simultaneously)

  • Two-lasers: can accommodate fluorophores excited by the
    HeNe (633nm) or Argon (488nm) lasers


DakoCytomation MoFlo:

  • High pressure/high speed sorting-i.e. pressures >30psi and sorting rates of approximately 25,000 cells/second.  Note:  Sorting rates and pressures will vary depending on cell type and sample preparation and may exceed the above mentioned values.

  • 4-way sorting

  • Single-cell sorting

  • 5-laser instrument: can accommodate most fluorophores available



 
     
 

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Flow Cytometry Core Facility  710 N. Fairbanks Court  Chicago, IL  60611
Phone:  (312) 908-1294   Fax:  (312) 908-1372   E-mail:  J-Nelson3@northwestern.edu
Last updated November, 2007  World Wide Web Disclaimer and University Policy Statements
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