A. THE BIOTECHNOLOGY FACILITY is located on both the Chicago
and Evanston campuses. It provides the following efficient, cost
effective and sophisticated technologies: synthesis and purification
of oligonucleotides and peptides, automated polymerase chain reaction
(PCR), peptide mapping, computer analysis of nucleic acid and
protein sequences, amino acid analysis of protein/peptide hydrolysates,
characterization of complex oligosaccharides and glycolipids by
high performance liquid chromatography (HPLC) and large scale
bacteria and fungi production. For more information contact James
Burrows, Assoc. Director, Searle 5-544, telephone 3-2144 or jburrows@nwu.edu
B. THE CELL IMAGING CENTER's purpose is to provide access
to equipment, technical support and training in light, confocal
and electron microscopy. Originally conceived by the Cell Biology
and Anatomy Department, the center has grown and expanded its
resources and techniques to serve the needs of a more comprehensive
Cell, Structural and Molecular biology Department, as well as
Lurie Cancer Center members who wish to take advantage of a variety
of microscopical and ancillary techniques. For more information
contact Robert Mihalik, Facility Manager, Ward 7-143, telephone
3-4445.
C. CENTRAL FACILITIES supports research by providing tissue culture media, animal sera, enzymes, and reagents at low cost to all NU labs and affiliated hospitals.
In addition, Central Facilities oversees and maintains all of
the major pieces of Lurie Cancer Center common equipment to support
the needs of Lurie Cancer Center members. The centralized media
preparation is under the auspices of one supervisor and one full time employee. This facility prepares
both standard and custom media and tissue culture reagents. It
also stocks Fisher and Gibco biochemicals. Promega restriction enzymes, and Promega, BRL, and QIAGEN molecular biology kits. All items are offered at costs lower than available from outside vendors. Full Members of the Lurie Cancer Center receive discounted pricing when purchasing products used to support peer reviewed funded research. For more information contact Todd Thompson, Manager, Olson 8402,
telephone 8-5927.
D. THE CYTOGENETICS FACILITY is located in the Prentice
Pavilion on the 15th floor and is designed to provide three types
of service: 1) clinical cytogenetics services and cancer cytogenetics; 2) laboratory and teaching
experiences for medical students, residents and fellows; and 3)
clinical and basic research in cytogenetics with emphasis on prenatal
diagnosis and cancer cytogenetics. For more information contact Maya
Thangavelu, Ph.D., Director, telephone 8-0991.
E. THE DNA SEQUENCE COMPUTATIONAL FACILITYprovides computational
support for DNA and protein sequence analysis. It provides software
resources to a large number of users who require access to very
large data bases of protein and nucleic acids sequences. The
facility provides software for editing and modifying sequences
as well as data bases for comparison and analysis. For more information
contact Alfonso Mondragon, Ph.D., Director, located on the Evanston
Campus, Hogan 2-100, telephone 708-491-7726 or A-MONDRAGON@nwu.edu.
F. FREEZERS containing restriction enzymes, PCR reagents,
and molecular biology reagents are situated in the Biotechnology
Facility on Searle 5-544. Contact Jim Burrows, Director, at 3-2144
to inquire about specific reagents. Reagent manufacturers include:
USB, Perkin-Elmer, and Gibco-BRL.
G. THE FLOW CYTOMETRY FACILITY (The Analytical and Quantitative
Cytology Facility) includes laboratories at Northwestern Memorial
Hospital and VA Lakeside Medical Center. The mission of the laboratories
is to provide instrumentation and oversight for research endeavors
involving analytical and quantitative cytology. The laboratories
are equipped with three flow cytometer instruments: a Coulter
PROFILE analyzer, a dual-laser Coulter ELITE analyzer/cell sorter
and a dual laser Coulter EPICS 753 analyzer/cell sorter. The
instruments are located in the Wesley Pavilion (rooms 536 and
539). For more information contact Charles L. Goolsby, Ph.D.,
Director, telephone 943-6600, ext. 718.
H. MONOCLONAL ANTIBODY FACILITYprovides investigators at Northwestern University and its affiliates services involved in the generation and production of monoclonal antibodies. The facility offers technical expertise in animal handling, cell culture and hybridoma technology. Services available include generation of Monoclonal Antibodies, antigen-specific screening, cell cloning, cell culture, isotype determination, ascities production, in vitro antibody production (Large quanities of antibody can be produced using the in vitro hollow fiber system, which is suitable for a variety of cell types in research applications including: bone marrow, lymphocytes, hybridomas, endothelial cells and recombinant proteins) For more information contact Lynn Doglio, located on the Evanston Campus, Hogan 1-150, telephone 708-467-2093.
I. MEDIA PRODUCTIONservices are supplied to most faculty
by Northwestern Memorial Hospital (facility director is David
Weil, telephone 8-6334 or dweil@nmh.org fax 8-2941 (Passavant, room 235). Services available include: comprehensive black and white adn color photography, clinical/portrait/location video and photographic services, medical illustration, electronic imaging. computer graphics and posters for conferences.
J. RESEARCH HISTOLOGY is a centralized resource for faculty
located in Northwestern Memorial Hospital. The Laboratory is
utilized for service oriented activities within the Pathology
Department and for research by NUMS investigators. The Laboratory
has three main objectives: to perform immunohistochemical procedures
for both service and research projects, to provide specialized
staining techniques, and to process, cut and stain tissues. In
situ hybridization is also being developed. The Lurie Cancer
Center plans to develop a Tumor Tissue Bank in the future if additional
resources become available. The current facility is located on
the second floor of the Passavant Pavilion. For more information
contact Sambasiva Rao, M.D., Director, telephone 3-8066.
K. THE TRANSGENIC FACILITY was established in the fall
of 1989, with the goal of helping Northwestern University faculty
and members of the broader research community to develop strains
of laboratory animals important in biomedical research by germ
line genetic transformation. The facility is capable of microinjecting
DNA into pronuclear stage embryos and then transferring these
embryos into the reproductive tracts of surrogate mothers. The
animals mainly used are mice and rats, but other animals are available
as required. For more information contact Phillip Iannaccone,
M.D., D.Phil., Director, or Lynn Doglio, Associate Director telephone 3-5232.
L. THE TWO-DIMENSIONAL (2-D) ELECTROPHORESIS FACILITY is
located on the eleventh floor of the Tarry Building in the Department
of Urology. This facility provides the service of all aspects
of 2-D electrophoresis to NUMS faculty. Separated proteins may
be visualized by staining or by autoradiography. Furthermore,
specific proteins can also be identified by Western blotting or
by sequencing following electrophoresis. Additionally, a computer-based
image analysis system is also available to extract information
from complex protein profiles and to compare differences among
various 2-D profiles. For more information contact Chung Lee,
Ph.D., Director, telephone 8-2916.
Last Updated: 10/10/95
Medical Administrative Operations, Northwestern University Medical School